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窖蛋白-1在不同肿瘤中发挥作用不同.本研究以小鼠肝癌细胞H22为研究对象,观察下调窖蛋白-1表达对H22细胞侵袭能力的影响,并探讨其可能的分子机制.利用RT-PCR和Western印迹法检测了窖蛋白-1在H22及小鼠正常肝细胞IAR20中的表达.结果显示,窖蛋白-1在H22中的表达高于其在IAR20中的表达,提示窖蛋白-1高表达可能与H22细胞恶性表型有关.RNA干扰和凝集素印记实验结果显示,窖蛋白-1-siRNA能够有效抑制窖蛋白-1mRNA和蛋白表达,并抑制细胞表面N-聚糖β1,6GlcNAc分支形成.Transwell细胞迁移和侵袭实验结果显示,与未转染组和siRNA对照组比较,转染窖蛋白-1-siRNA的H22细胞迁移和侵袭数目明显减少.本研究证明,下调窖蛋白-1表达可抑制H22细胞表面N-聚糖β1,6GlcNAc分支形成,从而抑制细胞迁移和侵袭能力.
Caveolin-1 plays a different role in different tumors. In this study, mouse hepatocellular carcinoma cell line H22 was studied to observe the effect of down-regulating caveolin-1 expression on the invasive ability of H22 cells, and to explore its possible molecular mechanism. Using RT- The expression of caveolin-1 in H22 and normal mouse hepatocytes IAR20 was detected by PCR and Western blot. The results showed that the expression of caveolin-1 in H22 was higher than that in IAR20, suggesting caveolin-1. High expression may be related to the malignant phenotype of H22 cells. RNA interference and lectin imprinting experiments show that caveolin-1 siRNA can effectively inhibit the expression of caveolin-1 mRNA and protein, and inhibit the cell surface N-glycan β1,6 GlcNAc branch The results of Transwell cell migration and invasion assay showed that the number of migration and invasion of H22 cells transfected with caveolin-1 siRNA was significantly reduced compared with untransfected and siRNA control groups. This study demonstrated that down-regulation of caveolin-1 expression. It can inhibit the formation of branching of N-glycan β1,6GlcNAc on H22 cells, thus inhibiting cell migration and invasion.