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目的:探讨高压氧对急性CO中毒大鼠脑内源性神经干细胞的影响,分析HBO治疗急性CO中毒脑损伤的机制。方法:建立急性CO中毒大鼠模型,给予高压氧(HBO)治疗后,H-E染色观察大鼠脑组织病理学变化,免疫组织化学方法检测大鼠脑内神经干细胞(nestin)和星形胶质细胞(GFAP)的表达。结果:H-E染色标本上,对照组脑内神经元形态正常,染毒组脑皮质出现大量变性坏死细胞,海马锥体细胞层稀疏,HBO组坏死细胞明显减少。免疫组化结果显示对照组nestin和GFAP表达数量形态均正常,染毒组nestin表达增加,但无统计学意义,GFAP形态数量发生改变,HBO组nestin表达明显增加,且在大脑皮层可见部分nestin阳性细胞和nestin-GFAP双阳性细胞;GFAP表达趋于正常。结论:急性CO中毒作为脑损伤因素可轻度激活大鼠脑内源性神经干细胞,并使星形胶质细胞增生变形、神经元变性坏死,HBO治疗可减轻星形胶质细胞损伤,明显激活内源性神经干细胞,并促使其增殖、迁移和分化。提示HBO可能通过激活神经干细胞起治疗作用。
Objective: To investigate the effect of hyperbaric oxygen on endogenous neural stem cells in acute CO poisoning rat brain and to analyze the mechanism of HBO treatment in acute CO poisoning brain injury. Methods: A rat model of acute CO poisoning was established. After hyperbaric oxygen (HBO) treatment, HE staining was used to observe the pathological changes of brain tissue in rats. Immunohistochemistry was used to detect the expression of nestin and astrocyte in rat brain (GFAP) expression. Results: In the H-E staining group, the neurons in the control group were normal in morphology. A large number of degenerated and necrotic cells appeared in the cerebral cortex of rats in the control group. The pyramidal cells were sparse in the hippocampus, and the number of necrotic cells in HBO group was significantly decreased. Immunohistochemistry showed that the number of nestin and GFAP in the control group was normal and the expression of nestin was increased in the exposed group, but there was no statistical significance. The number of GFAP changed, the expression of nestin in HBO group was significantly increased, and some nestin positive in the cerebral cortex Cells and nestin-GFAP double positive cells; GFAP expression tended to be normal. CONCLUSIONS: Acute CO poisoning may be a brain injury factor that may mildly activate endogenous neural stem cells in rat brain, degenerate and degenerate astrocytes and degeneration and necrosis of neurons. HBO treatment can reduce astrocyte injury and activate obviously Endogenous neural stem cells, and promote its proliferation, migration and differentiation. Hint HBO may play a therapeutic role by activating neural stem cells.