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目的研制新的流行性乙型脑炎传代细胞疫苗。方法将流行性乙型脑炎弱毒株ＳＡ１４１４２适应于Ｖｅｒｏ细胞，作纯化灭活疫苗，比较不同培养方法病毒在Ｖｅｒｏ细胞的增殖规律，对病毒的浓缩和纯化条件进行研究，并将所制备的疫苗按不同蛋白浓度免疫动物。结果发现普通转瓶培养，可长时间维持病毒的高滴度。在感染病毒后，以无牛血清的培养基替代含牛血清的培养基，不仅可维持病毒的高滴度增殖，而且可多次收获，确立了应用８％ＰＥＧ８０００浓缩病毒液，１５％～６０％的蔗糖密度梯度超速离心纯化的工艺，每剂量为０５μｇ纯化疫苗的中和抗体水平，即可达到与现有商品疫苗相一致的滴度。结论ＳＡ１４１４２株制备的Ｖｅｒｏ细胞纯化疫苗，有望成为一种新的反应轻、效价高的疫苗。
Objective To develop a new epidemic of Japanese encephalitis cell vaccine. Methods The attenuated strains of Japanese encephalitis SA14 14 2 adapted to Vero cells for purification of inactivated vaccine, compared with different culture methods in the proliferation of Vero cells in the virus, the virus concentration and purification conditions, and will The prepared vaccines were immunized at different protein concentrations. The results found that ordinary bottle culture, the virus can be maintained for a long time high titer. After the virus was infected, the medium without bovine serum was used instead of the medium containing bovine serum to not only maintain the high titer of the virus but also to harvest multiple times. The application of 8% PEG8000 concentrated virus solution and 15% ~ 60 % Sucrose density gradient ultracentrifugation purification process, each dose of 0 5μg purified vaccine neutralizing antibody levels, to achieve the same with the current commercial vaccine titer. Conclusion Vero cells purified vaccine prepared by SA14142 strain is expected to become a new vaccine with high response and light titer.