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目的 探讨卵巢癌患者腹水微量癌细胞中的端粒酶活性检测的临床意义。方法 采用以PCR技术为基础的TRAP方法检测卵巢癌腹水细胞标本中的端粒酶活性,并将检测结果与细胞学诊断结果进行比较。结果 对30例经病理学确诊的卵巢癌腹水细胞标本,采用细胞学检查和端粒酶活性检测发现,20例细胞学阳性腹水标本中,端粒酶阳性19例;7例细胞学阴性腹水标本中,端粒酶阳性3例;3例细胞学可疑腹水标本,其端粒酶活性检测均为阳性。细胞学诊断和端粒酶检测阳性率分别为66.67%(20/30)和83.33%(25/30)。结论 端粒酶活性检测可能在卵巢癌腹腔转移、腹水性质的鉴别诊断和疗后微小残存检测方面有重要辅助诊断价值。
Objective To investigate the clinical significance of detecting telomerase activity in ascites micro-cancer cells in patients with ovarian cancer. Methods The TRAP method based on PCR was used to detect the telomerase activity in ovarian ascites samples and the results were compared with the results of cytological diagnosis. Results The cytology and telomerase activity of 30 cases of pathologically confirmed ovarian ascites cells were detected in 20 cases of cytological positive ascites samples, 19 cases of telomerase positive; 7 cases of cytological negative ascites specimens , Telomerase positive in 3 cases; 3 cases of cytological suspicious ascites samples, the detection of telomerase activity were positive. The positive rates of cytology and telomerase detection were 66.67% (20/30) and 83.33% (25/30), respectively. Conclusion The detection of telomerase activity may have important diagnostic value in peritoneal metastasis of ovarian cancer, differential diagnosis of ascites and minimal residual after treatment.