论文部分内容阅读
目的:研究Cox-2蛋白在宫颈癌放疗及放化疗过程中的表达及其与肿瘤细胞凋亡的关系,以指导临床治疗。方法:60例患者随机分成2组,单纯放疗(radiotherapy RT)组30例给予10 Gy放疗,放化疗(synchronal radiochemotherapy CRT)组30例先行一次化疗,再给予10 Gy放疗,两组放疗前后均取活检,采用免疫组织化学法和脱氧核糖核酸转移酶介导的缺口末端标记(TUNEL)技术,分别检测30例单纯放疗和30例放化疗联合宫颈癌患者治疗前后宫颈肿瘤细胞凋亡率及Cox-2蛋白的表达。结果:在RT组和CRT组中治疗前Cox-2蛋白的表达分别为50%和53.33%,治疗后分别为30%和23.33%,表达阳性率治疗后较治疗前都有明显降低,CRT组治疗前后阳性率有统计学差异(P<0.05),但RT组治疗前后阳性率对比无统计学差异(P>0.05)。RT组放疗前和放疗10 Gy后,肿瘤细胞凋亡率分别为33.33%和66.67%,有统计学差异(P<0.05);CRT组治疗前和先行一次化疗再放疗10 Gy后,肿瘤细胞凋亡率分别为36.67%和93.33%,两者有统计学差异(P<0.01)。治疗后组间对比,放化疗组凋亡阳性率明显高于单纯放疗组,差异有统计学意义(P<0.05)。相关分析表明,肿瘤细胞中治疗前及治疗后细胞凋亡与Cox-2表达均呈显著等级负相关。结论:Cox-2可能通过调控肿瘤细胞的凋亡发挥作用,并在肿瘤恶性转化中可能起着重要作用。因此,检测Cox-2蛋白的表达有可能作为判断宫颈癌的生物学行为和临床预后的指标之一。
Objective: To study the expression of Cox-2 protein in cervical cancer during radiotherapy, radiotherapy and chemotherapy and its relationship with tumor cell apoptosis in order to guide clinical treatment. Methods: Sixty patients were randomly divided into two groups. Radiotherapy (RT) group was given 30 Gy radiotherapy. In the group of synchronal radiochemotherapy CRT, 30 patients were given chemotherapy first and then 10 Gy radiotherapy. Both groups were taken before and after radiotherapy Biopsy, immunohistochemistry and DNA nick end labeling (TUNEL) technique were used to detect the apoptosis rate of cervical neoplasm and the expression of Cox-1 in 30 patients with radiotherapy and 30 patients with radiotherapy and chemotherapy combined with cervical cancer before and after treatment, 2 protein expression. Results: The expressions of Cox-2 protein in RT group and CRT group were 50% and 53.33% respectively before treatment and 30% and 23.33% after treatment, respectively. The positive expression rate of Cox-2 protein in CRT group and CRT group was significantly lower than that before treatment There was a significant difference in the positive rate before and after treatment (P <0.05), but there was no significant difference between before and after treatment in RT group (P> 0.05). The apoptosis rates of tumor cells before radiotherapy and 10 Gy in RT group were 33.33% and 66.67%, respectively (P <0.05). Before CRT and 10 Gy after radiotherapy, the apoptosis of tumor cells The mortality rates were 36.67% and 93.33% respectively, with statistical difference (P <0.01). After treatment, the positive rate of apoptosis in radiotherapy and chemotherapy group was significantly higher than that of radiotherapy alone group, the difference was statistically significant (P <0.05). Correlation analysis showed that there was a significant negative correlation between apoptosis and Cox-2 expression in tumor cells before and after treatment. Conclusion: Cox-2 may play a role in the regulation of apoptosis of tumor cells and may play an important role in the malignant transformation of tumor. Therefore, the detection of Cox-2 protein expression may be used as an indicator of cervical cancer to determine the biological behavior and clinical prognosis.