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目的研究血管紧张素Ⅱ-1型受体(AGTR1)基因启动子区DNA甲基化水平与原发性高血压(EH)的相关性。方法选取在宁波市居住3代及以上的35~70岁汉族居民3 000人,分为新发病例组、既往病例组和对照组;按年龄、性别1∶1∶1匹配后有96对(共288人)进入病例对照研究。通过调查问卷、体格检查和实验室检测获取研究对象的基线资料及血生化指标。采用焦磷酸测序法检测AGTR1基因启动子区Cp G1~Cp G5位点的甲基化水平。采用条件Logistic回归模型对混杂因素进行校正,分析抗高血压药物治疗敏感的Cp G位点。结果三组研究对象的BMI、三酰甘油(TG)、空腹血糖(FPG)、高密度脂蛋白(HDL)、尿酸(UA)水平差异均有统计学意义(P<0.05)。条件Logistic回归分析结果显示,与对照组的Cp G1甲基化水平(9.66±5.45)%比较,新发病例组(6.74±4.32)%(OR=0.888,95%CI:0.792~0.995)和既往病例组(4.99±3.97)%(OR=0.454,95%CI:0.226~0.913)均偏低,而新发病例组与既往病例组间,未见有差异的Cp G位点(均P>0.05)。结论 AGTR1基因Cp G1的低甲基化是EH的影响因素,抗高血压药物治疗可能对AGTR1基因DNA甲基化水平无影响。
Objective To investigate the relationship between DNA methylation and essential hypertension (EH) in the promoter region of angiotensin Ⅱ type 1 receptor (AGTR1) gene. Methods A total of 3 000 Han residents aged 35-70 years old living in Ningbo for 3 generations or more were selected and divided into new cases, previous cases and control groups. There were 96 pairs A total of 288) into the case-control study. Through the questionnaire, physical examination and laboratory tests to obtain baseline data and blood biochemical indicators of the study. Pyrosequencing was used to detect the methylation level of CpG1 ~ CpG5 in AGTR1 promoter region. Conditioned Logistic regression models were used to correct for confounding factors and to analyze CpG sites sensitive to antihypertensive treatment. Results The BMI, triglyceride (TG), fasting plasma glucose (FPG), high density lipoprotein (HDL) and uric acid (UA) in the three groups were all significantly different (P <0.05). Logistic regression analysis showed that compared with the control group (9.66 ± 5.45)%, the newly diagnosed cases (6.74 ± 4.32)% (OR = 0.888, 95% CI: 0.792-0.995) (4.99 ± 3.97)% (OR = 0.454, 95% CI: 0.226-0.913), but there was no difference in CpG sites between the newly diagnosed group and the previous cases (all P> 0.05 ). Conclusion The hypothyroidism of AGTR1 gene Cp G1 is the influencing factor of EH. Antihypertensives treatment may not affect the DNA methylation level of AGTR1 gene.