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目的研究苏木醇提物对小鼠骨髓来源的树突状细胞(dendritic cell,DC)的IL-12 P40mRNA转录的影响。方法采用体外条件培养法得到小鼠骨髓来源树突状细胞,苏木醇提液浓度分别为5μg/ml、10μg/ml、20μg/ml。处理细胞后,扫描电镜观察、摄片,RT-PCR测定IL-12 P40mRNA的转录。结果苏木醇提物各组均可促进DCIL-12 P40的mRNA的表达。结论提示苏木醇提物对DC细胞分泌IL-12P40的促进作用,是通过增加IL-12 P40的mRNA转录实现的。
Objective To study the effect of alcohol extract of Hematoxylin on IL-12 P40 mRNA transcription in mouse bone marrow-derived dendritic cells (DCs). Methods The bone marrow-derived dendritic cells were obtained by in vitro culture. The concentrations of hematoxylin and eosin were 5μg / ml, 10μg / ml and 20μg / ml respectively. After the cells were treated, the transcription of IL-12 P40 mRNA was determined by scanning electron microscopy, radiography and RT-PCR. Results All the extracts of Hematoxylin and eosin could promote the expression of DCIL-12 P40 mRNA. Conclusions These results suggest that the promotion of IL-12P40 secretion by DCs is mediated by the increase of IL-12 P40 mRNA transcription.