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目的检测人不同时期烧伤创面渗液可溶性肿瘤坏死因子受体-I、II(sTNFR-I、sTNFR-II)和可溶性血管细胞黏附分子-I(sVCAM-I)与创面巨噬细胞数量,并分析相互间的关系。方法在换药时采用海绵吸取创面渗液,用免疫酶联法测定sTNFR-I、sTNFR-II、sVCAM-I,手术中取烧伤创面组织,切片HE染色和免疫组化染色标记CD68,计数巨噬细胞数量。结果烧伤创面渗液中sTNFR-I、sTNFR-II浓度及创面组织中的巨噬细胞数量在伤后的第2天显著增加,在烧伤1周后进一步提高,并在创面愈合前维持在较高水平。sVCAM-I在烧伤后即显著下降,并一直维持在低水平。创面渗液sTNFR-I、sTNFR-II、sVCAM-I浓度变化与巨噬细胞数量变化的相关系数分别为0.95、0.97、-0.37。结论sTNFR-I、sTNFR-II与巨噬细胞数量显著相关,提示深度烧伤创面愈合过程中sTNFR与巨噬细胞可能具有协同作用。
Objective To detect the number of soluble vascular cell adhesion molecule-I (sVCAM-I) and soluble vascular cell adhesion molecule-I (sTNFR-I, sTNFR- The relationship between each other. Methods During wound healing, sponge was used to infect wound exudate. STNFR-I, sTNFR-II and sVCAM-I were detected by enzyme-linked immunosorbent assay. Burned wounds were obtained from the wounds. HE staining and immunohistochemistry were used to stain CD68. The number of macrophages. Results The concentration of sTNFR-I, sTNFR-II in wound exudate and the number of macrophages in wound tissue increased significantly on the second day after burn injury and further increased one week after burn injury and remained high before wound healing Level. sVCAM-I significantly decreased after burn, and has been maintained at a low level. The correlation coefficients of changes of sTNFR-I, sTNFR-II and sVCAM-I concentration with the number of macrophages were 0.95,0.97 and -0.37 respectively. Conclusion sTNFR-I and sTNFR-II are significantly correlated with the number of macrophages, suggesting that sTNFR and macrophages may have a synergistic effect during the healing of deep burn wounds.