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采用农杆菌介导法将含有复合OM启动子和增强子Ω因子的外源基因pBinMoBc导入冀棉20中.转化再生株经分子检测表明,外源基因已整合到转基因植株基因组内,并得到了表达,转化外源基因插入拷贝数是1~2个.转化后代材料经6~7代选育为纯合品系2001pb-3.2001pb-3抗虫性较好,每公顷产皮棉1534.5kg,比对照增产18.68%.图5表3参4
The exogenous gene pBinMoBc containing complex OM promoter and enhancer omega-factor was introduced into jm cotton 20 by agrobacterium-mediated method.The molecular analysis showed that the exogenous gene has been integrated into the genome of the transgenic plant and has been obtained Expression and transformation of exogenous gene insertion copy number is 1 to 2. The generation of transformed progeny after 6 to 7 generations of homozygous lines 2001pb-3.2001pb-3 insect resistance, lint yield of 1534.5kg per hectare, compared with the control Yield 18.68%. Figure 5 Table 3 Senate 4