论文部分内容阅读
甜菜碱醛脱氢酶(betaine aldehyde dehydrogenase,BADH)是合成甜菜碱的关键酶,广泛用于植物的耐盐转基因研究。为获得耐盐性提高的棉花植株,本研究通过花粉管通道法将山菠菜甜菜碱醛(BADH)基因转化到陆地棉品种——中棉所35。经卡那霉素田间抗性鉴定、标记基因NPT-Ⅱ和目标基因BADH的PCR检测,以及Southern杂交检测,结果表明BADH已整合到棉花的基因组中,并获得转标记基因NPT-Ⅱ和目标基因BADH棉株5株。通过对T2种子在0.6%Na Cl盐池发芽实验结果表明,转化植株的出苗率高达63.4%,对照材料出苗率2.4%,转化植株耐盐性较对照有明显提高。本研究获得转基因植株可作为抗逆育种的种质材料。
Betaine aldehyde dehydrogenase (betaine aldehyde dehydrogenase, BADH) is a key enzyme in the synthesis of betaine and is widely used in plant salt tolerance transgenic research. In order to obtain cotton plants with increased salt tolerance, we studied the genetic transformation of BADH gene into cotton cultivar CIMC by the pollen tube pathway. The results showed that BADH was integrated into the genome of cotton and the transgenic marker NPT-Ⅱand the target gene were obtained by PCR analysis of the marker gene NPT-Ⅱ and target gene BADH and Southern blotting. BADH five cotton plants. The results of germination of T2 seeds in 0.6% NaCl salt pool showed that the emergence rate of the transformed plants was as high as 63.4% and that of the control material was 2.4%. The salt tolerance of the transformed plants was significantly higher than that of the control. The transgenic plants obtained in this study can be used as germplasm materials for resistance breeding.