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通过室内及田间大豆不同生育期接种试验证明，籽粒感染灰斑病的关键时期是Ｒ３－Ｒ５期．Ｒ２期以前侵染不造成籽粒斑驳，据此提出一次防治大豆籽粒灰斑病的关键时期为Ｒ２－Ｒ４期．
Inoculation experiments of indoor and field soybean at different growth stages demonstrated that the key period of grain rust infection was R3-R5. The R2 period before infection did not cause grain mottling, thus proposed a key period for the control of soybean gray leaf spot is R2-R4 period.