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以菜薹—青花菜异源双二倍体(AACC)为桥梁亲本,通过与亲本菜薹(AA)回交获得了菜薹—青花菜种间异源三倍体AAC。该三倍体总体形态介于菜薹与青花菜之间,生长势较强,经根尖染色体鉴定,具有预期的染色体数2n=3x=29;花粉生活力及单个花蕾的花粉量都明显的低于菜薹和青花菜;减数分裂时期染色体行为复杂,在中期Ⅰ、后期Ⅰ、中期Ⅱ和后期Ⅱ存在落后染色体,后期Ⅰ染色体不均等分离,有15/14、16/13等多种分离方式,形成染色体数不等的配子。
Heterotopic triploid AAC was screened out from the combination of Alopecurus barbata-Alopecurus versicolor heterodundiploid (AACC) and their parental parents by backcrossing with the parent flowering Chinese cabbage (AA). The overall morphology of the triploid between the cabbage and broccoli between the strong growth potential, identified by the apical chromosome, with the expected chromosome number 2n = 3x = 29; pollen viability and individual buds of the amount of pollen were significant Lower than the flowering cabbage and broccoli; chromosomes during meiosis were complex, with backward chromosomes in metaphase I, metaphase II, metaphase II and metaphase II, and late metaphase I with unequal segregation of chromosomes 15/15, 16/13 Separation methods, the formation of chromosomes ranging from gametes.