[Objective] To research on the protective effects of total flavonoids in Polygonum perfoliatum L. on the acute liver injury of mice induced by CCl4 and its action mechanism. [Methods]Flavonoids were extracted from P. perfoliatum by chemical method. Acute liver injury model of mice was established by intraperitoneal injection of CCl4 peanut oil. The biochemical indexes of ALT,AST,TNF-α,IL-1β and IL-6in mice serum were detected. Histopathological examination of liver tissue was carried out. [Results] Flavonoids in P. perfoliatum restricted the ALT,AST,TNF-α,IL-1β and IL-6 activities in mice serum with acute liver injury( P < 0. 01,P < 0. 05). Results of histopathological examination also showed that flavonoids significantly improved the liver injury of mice. [Conclusions] Flavonoids in P. perfoliatum showed protective effects of acute liver injury of mice induced by CCl4; and its mechanism might be related with anti-inflammation. [Objective] To research on the protective effects of total flavonoids in Polygonum perfoliatum L. on the acute liver injury of mice induced by CCl4 and its action mechanism. [Methods] Flavonoids were extracted from P. perfoliatum by chemical method. Acute liver injury model of mice was established by intraperitoneal injection of CCl4 peanut oil. The biochemical indexes of ALT, AST, TNF-α, IL-1β and IL-6in in mice were detected. [Results] Flavonoids in P. perfoliatum restricted the ALT, AST, TNF-α, IL-1β and IL-6 activities in mice serum with acute liver injury (P <0.01, P <0.05). Results of histopathological examination also showed that flavonoids significantly improved the liver injury of mice. [Conclusions] Flavonoids in P. perfoliatum showed protective effects of acute liver injury of mice induced by CCl4; and its mechanism might be related with anti-inflammation.