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目的通过动物实验观察超微结构研究预防椎板切除术后硬膜外粘连的有效材料和方法。方法取卡波姆(Carborner)与丹参(SMB),制成凝胶、丹参凝胶。36只大白兔随机分为4 组;切除L3、L6椎板后造成10 mm×5 mm椎板缺损区。A组:空白对照组;B组:凝胶对照组;C 组:透明质酸钠(HA)组;D组:丹参凝胶组。术后4周时标本进行透射电镜观察,4、6、8周L3、L6 两节段标本进行瘢痕厚度的计算机图像分析。结果瘢痕厚度A、B、C组与D组有明显差异。F 值分别为128．657、152．246、80．891;电镜观察:A、B、C组均可见增殖活跃的成纤维细胞,D组成纤维细胞增值不活跃。结论丹参凝胶具有良好的预防椎板切除术后硬膜外粘连的作用,丹参可抑制成纤维细胞增生。
Objective To observe the effect of ultrastructural studies on the prevention of epidural adhesion after laminectomy by animal experiments. Methods Carbopol and Salvia miltiorrhiza (SMB) were used to prepare gels and salvia miltiorrhiza gels. Thirty-six rabbits were randomly divided into four groups; L3 and L6 laminae were resected to create a laminar defect of 10 mm x 5 mm. A group: blank control group; B group: gel control group; C group: sodium hyaluronate (HA) group; D group: Danshen gel group. At 4 weeks postoperatively, the specimens were observed by transmission electron microscopy. Computerized image analysis of the thickness of the scars was performed on the L3 and L6 segments at 4, 6, and 8 weeks. Results There were significant differences in scar thickness between group A, B and C and group D. The F values were 128.657, 152.246, and 80.891, respectively. Electron microscopy showed that the proliferating fibroblasts were seen in groups A, B, and C, and the D-component fibroblasts were inactive. Conclusion Salvia miltiorrhiza has a good effect in preventing epidural adhesion after laminectomy. Salvia miltiorrhiza can inhibit the proliferation of fibroblasts.