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目的探讨非小细胞肺癌(NSCLC)患者呼出气冷凝液中核内不均一核糖核蛋白(hnRNP)A2/B1检测价值。方法收集NSCLC患者37例为NSCLC组,其中鳞癌17例,腺癌20例,TNM分期的Ⅰ期~Ⅱ期12例,Ⅲ期~Ⅳ期25例,另收集同期健康体检者25例为对照组,采集空腹血清和呼出气冷凝液(EBC)标本,采用ELISA方法检测血清及EBC中hnRNP A2/B1。结果 NSCLC组血清、EBC中hnRNP A2/B1水平均高于对照组,差异有统计学意义(P<0.05)。鳞癌组和腺癌组血清hnRNP A2/B1水平差异无统计学意义(P<0.05),鳞癌组EBC中hnRNP A2/B1水平高于腺癌组,差异有统计学意义(P<0.01)。Ⅲ期~Ⅳ期血清hnRNP A2/B1高于Ⅰ期~Ⅱ期,差异有统计学意义(P<0.05),Ⅰ期~Ⅱ期EBC中hnRNP A2/B1水平高于对照组,差异有统计学意义(P<0.05)。结论检测血液及EBC中hnRNP A2/B1水平对肺癌有辅助诊断价值,其中EBC中hnRNP A2/B1水平检测对早期肺癌的诊断有一定临床意义。
Objective To investigate the detection of heterogeneous nuclear ribonucleoprotein (hnRNP) A2 / B1 in exhaled breath condensate in patients with non-small cell lung cancer (NSCLC). Methods Totally 37 NSCLC patients were enrolled as NSCLC group, including 17 squamous cell carcinoma, 20 adenocarcinoma, 12 stage Ⅰ ~ Ⅱ TNM stage, 25 stage Ⅲ ~ Ⅳ stage and 25 healthy control subjects in the same period Groups, fasting serum and exhaled breath condensate (EBC) specimens were collected, and serum and EBC hnRNP A2 / B1 were detected by ELISA. Results The levels of hnRNP A2 / B1 in serum and EBC in NSCLC group were significantly higher than those in control group (P <0.05). The levels of hnRNP A2 / B1 in squamous cell carcinoma and adenocarcinoma group were not significantly different (P <0.05), while the level of hnRNP A2 / B1 in squamous cell carcinoma group was higher than that in adenocarcinoma group (P <0.01) . The level of hnRNP A2 / B1 in stage Ⅲ-Ⅳ was higher than that in stage Ⅰ-Ⅱ, and the difference was statistically significant (P0.05). The level of hnRNP A2 / B1 in stage Ⅰ-Ⅱ was higher than that in control group Significance (P <0.05). Conclusions The detection of hnRNP A2 / B1 in blood and EBC is of value in the diagnosis of lung cancer. The detection of hnRNP A2 / B1 in EBC has some clinical significance in the diagnosis of early stage lung cancer.