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探索了西宁市鱼类产品中孔雀石绿与结晶紫的高效液相色谱荧光检测方法,方法在0.1ng/g—00ng/g范围内具有很好的线性关系,相关系数r为0.9998.定量限为0.5μg/kg。采用不同的鱼类产品进行验证,孔雀石绿的回收率在88%—94%之间,相对标准偏差(RSD)为3.15%—4.87%。结晶紫的回收率在94%—108%,相对标准偏差(RSD)为2.54%—4.05%。本法提取快捷、简便,适用于大批量的抽样检测,可检测出鱼类样品中痕量的孔雀石绿,为水产养殖提供可行的监测手段,为鱼类中孔雀石绿的检测标准的制定提供依据。
A high performance liquid chromatography (HPLC) fluorescence detection method of malachite green and crystal violet in fish products of Xining has been explored. The method has a good linear relationship in the range of 0.1ng / g-00ng / g with a correlation coefficient of 0.9998. 0.5 μg / kg. Using different fish products for validation, the recovery of malachite green was between 88% and 94% with a relative standard deviation (RSD) of 3.15% -4.87%. The recovery rate of crystal violet is 94% -108%, and the relative standard deviation (RSD) is 2.54% -4.05%. This method is fast, simple, suitable for large-scale sampling and testing, can detect trace amounts of malachite green in fish samples, provide feasible monitoring means for aquaculture, and set the standard for the detection of malachite green in fish Provide evidence.