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为了研究人参皂甙-Rb_2对蛋白质生物合成的影响,测定了用药前后由链脲佐菌素诱发的糖尿病小鼠(DM)和正常小鼠(NDM)血清中蛋白质的变化。同时采用〔~(14)C〕标记亮氨酸放射结合测定法,测定了在肝脏细胞的亚显微结构中蛋白质的合成水平。与NDM组比较,DM组小鼠血清总蛋白及白蛋白水平显著下降,进入蛋白中放射性底物在减少,在肝脏细胞的胞浆、细胞核及微粒体片断中,与蛋白结合的放射性底物明显减少,但在线粒体和溶胶体中无明显变化。说明糖尿病小鼠与正常小鼠相比,蛋白质的生物合成功能下降。当DM组小鼠连续3d腹腔注射人参皂甙-Rb_2(剂量10mg/d)后,血清总
In order to study the effect of ginsenoside-Rb 2 on protein biosynthesis, the changes in serum of diabetic mice (DM) and normal mice (NDM) induced by streptozotocin before and after treatment were determined. Simultaneously, the [~(14)C]-labelled leucine radio-binding assay was used to determine the level of protein synthesis in the submicroscopic structure of liver cells. Compared with the NDM group, serum total protein and albumin levels in the DM group were significantly decreased, and radioactive substrates in the entry protein were decreased. In the liver cell cytoplasm, nucleus and microsomal fragments, the radioactive substrate bound to the protein was significantly Decreased, but no significant changes in mitochondria and sol bodies. This shows that the biosynthesis function of protein is decreased in diabetic mice compared with normal mice. When mice in DM group received intraperitoneal injection of ginsenoside-Rb 2 (dose 10 mg/d) for 3 days, serum total