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本文用反相高效液相色谱法,采用离子抑制技术分离了丹参注射剂中三种有效成分:丹参素、原儿茶醛、原儿茶酸。并用外标法测定了它们在丹参注射液和复方丹参注射液中的含量。考察了Waters公司分析柱(3.9×300 mm)和径向加压柱(8×100 mm),以及国产色谱柱(5×150mm)分析样品,均能获得满意的结果。比较了用多通道紫外吸收检测器和254nm固定波长紫外检测器测定样品时灵敏度的差异。
In this paper, three effective components of Danshensu injection were isolated by reversed-phase high performance liquid chromatography with ion suppression technology: Danshensu, protocatechuic aldehyde, protocatechuic acid. The content of Salvia miltiorrhiza injection and compound salvia miltiorrhiza injection was determined by external standard method. Waters analysis columns (3.9×300 mm) and radial pressure columns (8×100 mm), as well as home-made column (5×150 mm) samples, were investigated and satisfactory results were obtained. The difference in sensitivity when using multi-channel UV absorbance detectors and 254 nm fixed-wavelength UV detectors was compared.