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目的:研究下坡跑对生长期去卵巢后骨质疏松小鼠骨形成蛋白BMP-2及其信号转导蛋白Smad1/5和靶基因Runx2基因表达的影响。方法:24只4周龄雌性C57BL/6小鼠,适应性喂养1周后随机分为安静去卵巢组(C+OVX组)、运动去卵巢组(D+OVX组)和安静假手术组(C+SHAM组)3组。D+OVX组进行8周下坡跑,训练结束后C+OVX组和D+OVX组行去卵巢手术,C+SHAM组行假手术。术后正常喂养6周处死小鼠,采用RT-PCR检测骨BMP-2、Smad1/5和Runx2 mRNA表达;采用比色法检测血液生化指标;使用Hologic Discovery A骨密度仪检测前肢骨BMD。结果:与C+OVX组相比,D+OVX组BMP-2(P<0.01)、Smad1(P<0.05)、Smad5(P<0.01)和Runx2(P<0.01)mRNA表达显著上调,磷离子浓度和BMD显著升高(P<0.05),钙离子浓度显著降低(P<0.05)。与C+SHAM组相比,D+OVX组Runx2和Smad1mRNA表达显著下调(P<0.05),ALP活性显著降低(P<0.05),磷离子浓度显著升高(P<0.05)。与C+SHAM组相比,C+OVX组BMP-2(P<0.05)、Runx2(P<0.01)和Smad5(P<0.01)mRNA表达显著下调,ALP活性(P<0.05)和BMD(P<0.01)显著降低,磷离子浓度显著升高(P<0.01)。结论:BMP-2及其信号转导蛋白Smad1/5、靶基因Runx2基因表达水平较高可能是促进骨形成的生物学机制,下坡跑上调生长期去卵巢后小鼠BMP-2、Smad1/5和Runx2基因表达,可能与早期运动训练防治骨质疏松的机制有关。
Objective: To study the effects of downhill running on bone morphogenetic protein-2 (BMP-2), Smad1 / 5 and Runx2 mRNA expression in ovariectomized osteoporotic mice. Methods: Twenty-four four-week-old female C57BL / 6 mice were randomly divided into three groups: C group, OVX group, D + OVX group, and sham-operation group C + SHAM group) 3 groups. D + OVX group for 8 weeks downhill training, C + OVX group and D + OVX group ovariectomy after training, C + SHAM group sham operation. Mice were sacrificed 6 weeks after normal feeding. The expression of BMP-2, Smad1 / 5 and Runx2 mRNA were detected by RT-PCR. The blood biochemical parameters were detected by colorimetric assay. The BMD of forelimb bone was detected by Hologic Discovery A bone density meter. Results: Compared with C + OVX group, the mRNA expression of BMP-2 (P <0.01), Smad1 (P <0.05), Smad5 (P <0.01) and Runx2 (P <0.01) Concentration and BMD increased significantly (P <0.05), and calcium concentration decreased significantly (P <0.05). Compared with C + SHAM group, Runx2 and Smad1 mRNA expressions in D + OVX group were significantly decreased (P <0.05), ALP activity was significantly decreased (P <0.05) and phosphorus ion concentration was significantly increased (P <0.05). Compared with C + SHAM group, mRNA expression of BMP-2 (P <0.05), Runx2 (P <0.01) and Smad5 (P <0.01) <0.01), and the concentration of phosphorus increased significantly (P <0.01). Conclusion: The high expression of BMP-2, Smad1 / 5 and Runx2 may be the biological mechanism of promoting bone formation. Downhill running up-regulates the expression of BMP-2 and Smad1 / 5 and Runx2 gene expression may be related to the mechanism of early exercise training to prevent and treat osteoporosis.