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目的:研究小鼠病毒性心肌炎(VMC)模型中microRNA-21(miR-21)和microRNA-146b(miR-146b)基因表达,探究其在VMC发病中的变化及意义。方法:用柯萨奇病毒B3(CVB3)建立Balb/c VMC小鼠模型,实验组注射100TCID 50病毒液0.1ml,对照组注射等量磷酸盐缓冲液。在注射的第0、1、2、4周分别采用实时定量PCR法检测2组小鼠心肌组织miRNA-21和miR-146b表达水平。结果:与对照组比较,实验组小鼠心肌组织miR-21和miR-146b水平自第1周开始稳定表达,第2周时达峰值,并至少维持至第4周;实验组各时间点的表达量均高于对照组相应时间点(均P<0.05)。结论:miR-21和miR-146b在VMC小鼠心肌组织中表达明显增高,提示miR-21和miR-146b可能参与了VMC的发病过程。
OBJECTIVE: To study the expression of microRNA-21 (miR-21) and microRNA-146b (miR-146b) genes in viral myocarditis (VMC) in mice and explore its significance in the pathogenesis of VMC. Methods: The Balb / c VMC mouse model was established with Coxsackievirus B3 (CVB3). The experimental group was injected with 0.1ml of 100TCID 50 virus solution and the control group with the same amount of phosphate buffer solution. Real-time quantitative PCR was used to detect the expression of miRNA-21 and miR-146b in myocardium of 2 groups at 0, 1, 2, and 4 weeks after injection. Results: Compared with the control group, the levels of miR-21 and miR-146b in the myocardium of experimental mice were stable from the first week, and peaked at the second week, and remained at least until the fourth week. The expression of miR-21 and miR- Higher than the control group at the corresponding time points (all P <0.05). Conclusion: The expression of miR-21 and miR-146b in myocardium of VMC mice was significantly increased, suggesting that miR-21 and miR-146b may be involved in the pathogenesis of VMC.