论文部分内容阅读
利用耐高温酵母GXASY-10菌株对木薯粉同步糖化(SSF)法生产高浓度乙醇的发酵条件进行了优化。在单因素实验的基础上,首先应用Plackett-Burman试验设计筛选影响酒精高温高浓度发酵的重要参数,采用最陡爬坡实验逼近最大酒精生产区域后,利用Box-Behnken设计确定重要参数的最佳水平。筛选结果表明,影响酒精产量的重要参数是液化时间、糖化酶用量和初始木薯粉(底物)浓度。最佳工艺条件为:液化时间为35min,糖化酶添加量为1.21AGU/g底物,底物浓度为37.62%。20L发酵罐在此条件下(发酵温度37℃,转速100r/min)经过48h发酵,酒精浓度可达16.07%(V/W)。优化条件与初始条件相比较,酒精浓度提高了33%。
The fermentation conditions of high concentration ethanol produced by Simultaneous Saccharification (SSF) of cassava flour were optimized by using high temperature resistant yeast strain GXASY-10. Based on the single-factor experiments, the important parameters influencing the high-temperature and high-temperature fermentation of alcohol were first screened by the Plackett-Burman design. After the steepest ascent experiment approached the maximum alcohol production area, the Box-Behnken design was used to determine the most important parameters Level. Screening results show that the important parameters that affect the yield of alcohol are the liquefaction time, the amount of glucoamylase and the initial concentration of tapioca (substrate). The optimum conditions were as follows: liquefaction time was 35min, glucoamylase dosage was 1.21AGU / g substrate, substrate concentration was 37.62%. 20L fermenter under this condition (fermentation temperature 37 ℃, speed 100r / min) after 48h fermentation, alcohol concentration up to 16.07% (V / W). Optimization conditions Compared with the initial conditions, the alcohol concentration increased by 33%.