论文部分内容阅读
目的建立回生第一丹胶囊中血竭高氯酸盐含量测定方法。方法回生第一丹胶囊经3%磷酸甲醇振摇提取后,滤过,定容,以乙晴-0.05mol/L磷酸二氢钠溶液(50:50)以流动相,拄温35℃,检测波长:440nm,以RP-HPLC检测其中血竭高氯酸盐的含量。结果结性范围0.0254-0.538mg/ml,r2=0.9974,平均回收率92.65%,RSD=2.2%。结论本法简单,快捷,重复性良好,可用于回生第一丹胶囊中血竭高氯酸盐含量的质量控制。
Objective To establish a method for determination of the content of perchlorate in blood of Dangsheng Dandan Capsules. METHODS: The retrograded first Dan capsule was extracted with 3% phosphoric acid and methanol, filtered, and then transferred to a constant volume. The mobile phase consisted of acetonitrile-0.05mol/L sodium dihydrogen phosphate solution (50:50) and the reaction temperature was 35°C. Wavelength: 440 nm. The content of dragon’s blood perchlorate was measured by RP-HPLC. Results The conjunctive range was 0.0254-0.538mg/ml, r2=0.9974, the average recovery rate was 92.65%, RSD=2.2%. Conclusion This method is simple, rapid and reproducible. It can be used to control the quality of perchlorate in blood of Dangsheng Dandan capsules.