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目的:探讨急性白血病患者(AL)化疗前后外周血白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)、血管内皮生长因子(VEGF)水平变化及与临床预后的关系。方法:选2010年3月-2013年12月在我院行化疗治疗的53例AL患者(AL组),并同期抽选我院47例健康体检者为对照组,采用放射免疫以及酶联法检测两组外周血IL-2、TNF-α和VEGF水平,并探讨AL患者化疗前后外周血IL-2、TNF-α、VEGF水平变化及与临床预后的关系。结果:AL组化疗前外周血TNF-α、VEGF水平明显高于对照组(P<0.05),IL-2水平明显低于对照组(P<0.05)。AL组化疗6个月后外周血IL-2、TNF-α和VEGF含量与化疗前比较差异均有统计学意义(P<0.05)。AL组化疗6个月后长期缓解未复发者42例、复发者11例;复发患者的外周血TNF-α、VEGF水平较未复发组高,IL-2较未复发组低,组间差异具有统计学意义(P<0.05)。结论:检测AL患者的外周血IL-2、TNF-α、VEGF含量可作为诊断及判断预后的重要指标。
Objective: To investigate the changes of peripheral blood interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and vascular endothelial growth factor (VEGF) in patients with acute leukemia before and after chemotherapy . Methods: A total of 53 patients with AL (AL) who underwent chemotherapy in our hospital from March 2010 to December 2013 were enrolled. 47 healthy people in our hospital were selected as the control group by radioimmunoassay and enzyme-linked immunosorbent assay The level of IL-2, TNF-α and VEGF in the two groups were detected, and the changes of IL-2, TNF-α and VEGF in AL patients before and after chemotherapy were also analyzed. Results: The levels of TNF-α and VEGF in AL group were significantly higher than those in control group (P <0.05), while the levels of IL-2 in AL group were significantly lower than those in control group (P <0.05). The levels of IL-2, TNF-α and VEGF in peripheral blood in AL group after 6 months of chemotherapy were significantly different from those before chemotherapy (P <0.05). In AL group, there were 42 cases with long-term non-recurrence and 11 cases with recurrence after long-term chemotherapy. The levels of TNF-α and VEGF in peripheral blood were higher in patients with recurrent disease than in non-recurrent group and lower in IL-2 than in non-recurrent group Statistical significance (P <0.05). Conclusion: Detection of IL-2, TNF-α and VEGF in peripheral blood of patients with AL can be used as an important index for diagnosis and prognosis.