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目的探讨巴豆醛暴露对雄性大鼠的肝脏损伤。方法选取SPF级健康雄性Wistar大鼠40只,随机分成高剂量组、中剂量组、低剂量组和对照组4个组,每组10只。巴豆醛经口灌胃分别给予高剂量组8.44 mg/kg、中剂量组4.22 mg/kg、低剂量组2.11 mg/kg,大鼠灌胃量为1 ml/100 g体重。每天灌胃一次,连续染毒28天后处死动物。取外周血进行血生化检验;ELISA法检测血清中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性;取大鼠主要脏器,称重并计算脏器系数,常规HE染色制作病理标本,观察肝脏组织病理学改变。结果与对照组相比,高剂量组大鼠体重增重减少,肝脏脏器系数明显增高,且随着染毒剂量的增加,肝脏系数有增高的趋势;血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)水平明显增加,高剂量组和中剂量组的MDA含量升高,SOD含量降低,高剂量组的GSH-PX含量降低,差异均有统计学意义(P<0.05);与对照组相比,心脏系数、脾脏系数、肾脏系数及血清中总胆红素(TBIL)、尿素(BUN)、尿酸(UA)、肌酐(CRE)、葡萄糖(GLU)、甘油三酯(TG)、总胆固醇(CHOL)水平无明显变化,差异无统计学意义(P>0.05);病理组织切片显示大鼠肝组织有炎性损伤。结论巴豆醛暴露对雄性大鼠有肝损伤作用。
Objective To investigate the liver damage induced by crotonaldehyde in male rats. Methods Forty SPF healthy male Wistar rats were randomly divided into 4 groups: high dose group, middle dose group, low dose group and control group, with 10 in each group. The oral administration of crotonaldehyde to high dose group (8.44 mg / kg), middle dose group (4.22 mg / kg), low dose group (2.11 mg / kg) and rats intragastrically to 1 ml / 100 g body weight respectively. Gavage once a day, continuous exposure to animals after 28 days. Peripheral blood was taken for blood biochemical tests; serum malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activity were measured by ELISA; , Weighed and calculated organ coefficient, routine HE staining pathological specimens were observed liver histopathological changes. Results Compared with the control group, the body weight gain of rats in the high-dose group decreased and the coefficient of liver organ increased significantly. The hepatic coefficient increased with the increase of the dose of the drug. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (P <0.05). Compared with the control group (P <0.05), the levels of AST and AST in the high-dose group and middle-dose group were significantly increased (P> 0.05), the ratio of heart, spleen, kidney, TBIL, BUN, UA, CRE, GLU, TG and total cholesterol (CHOL) did not change significantly, the difference was not statistically significant (P> 0.05); pathological tissue sections showed inflammatory injury in rat liver tissue. Conclusion Crotonaldehyde exposure has the effect of liver injury in male rats.