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目的 :研究 cag E在幽门螺杆菌 (H elicobacter pylori,Hp)感染的不同胃肠疾病患者中的分布及其与 Hp感染相关疾病的关系。 方法 :合成 cag EU 1- cag EU 2和 cag E3- cag E4两组引物 ,应用聚合酶链反应 (PCR)法扩增 14 5株临床分离培养的Hp菌株 cag E片段。 结果 :Hp临床菌株的 cag EU 1- cag EU 2 PCR产物总检出率为 75 .9%(110 / 14 5 ) ,慢性胃炎、十二指肠溃疡、胃溃疡、复合溃疡中检出率分别为 6 9.4 %、85 .4 %、76 .5 %、75 .0 %,溃疡组略高于胃炎组 ,但差异无显著性 (P>0 .0 5 )。cag E3- cag E4总检出率为 4 2 .1%(6 1/ 14 5 ) ,慢性胃炎、十二指肠溃疡、胃溃疡、复合溃疡中检出率分别为 38.9%,4 7.9%,35 .3%,5 0 %,差异无显著性 (P>0 .0 5 ) ;cag E总检出率为 79.3%(115 / 14 5 )。 结论 :cag E在不同的消化道疾病患者感染的Hp中均有较高的检出率 ,在不同疾病中的分布无特异性 ,cag E尚不能单独作为与某种疾病相关的致病相关基因
Objective: To investigate the distribution of cag E in patients with different gastrointestinal diseases infected with Helicobacter pylori (Hp) and its relationship with Hp infection-related diseases. Methods: Two sets of primers of cag EU 1-cag EU 2 and cag E3-cag E4 were synthesized and amplified by polymerase chain reaction (PCR). Results: The overall detection rate of cag EU 1-cag EU 2 PCR product was 75.9% (110/14 5) in Hp clinical isolates. The detection rates of chronic gastritis, duodenal ulcer, gastric ulcer and complex ulcer were respectively 6 9.4%, 85.4%, 76.5% and 75.0% respectively. The ulcer group was slightly higher than the gastritis group, but the difference was not significant (P> 0.05). The overall detection rate of cag E3-cag E4 was 42.1% (61/14 5). The detection rates of chronic gastritis, duodenal ulcer, gastric ulcer and complex ulcer were 38.9%, 49.9% 35.3% and 50%, respectively, with no significant difference (P> 0.05). The overall detection rate of cag E was 79.3% (115/14 5). CONCLUSION: cag E has high detection rate in Hp infected by different digestive tract diseases, and has no specific distribution in different diseases. Cag E can not be used as a disease-related gene associated with certain diseases alone