论文部分内容阅读
目的研究叶酸代谢酶基因多态MTHFRC677T、MTHFRA1298C、MTRA2756G和MTRRA66G及其联合作用与结直肠癌(CRC)易感性的关系。方法采用聚合酶链-限制性片段长度多态性方法,检测140例CRC患者和343例正常对照者的叶酸代谢酶基因多态,采用非条件Logistic回归模型和似然比检验,分析各多态及其联合作用与CRC的关系。结果MTR2756G等位基因携带者患CRC的风险是野生型纯合子(AA)的2倍(95% CI为1．22～3．40)。与同时是MTHFR1298AA和MTR2756AA基因型者相比,同时是MTHFRl298AA和MTR2756AG/GG基因型者患CRC的风险显著升高(OR=2．57,95%CI为1．42～4．65),两者之间存在联合作用(P=0．04)。结论MTR2756G等位基因可能是CRC的危险因素,MTHFRA1298C和MTRA2756G之间存在联合作用。
Objective To study the relationship between the polymorphisms of the folic acid metabolic enzyme gene MTHFRC677T, MTHFRA1298C, MTRA2756G and MTRRA66G and their susceptibility to colorectal cancer (CRC). Methods Polymerase chain-restriction fragment length polymorphism was used to detect the polymorphism of folic acid metabolic enzyme gene in 140 CRC patients and 343 normal controls. Unconditional Logistic regression model and likelihood ratio test were used to analyze the polymorphisms. And its association with CRC. Results The risk of CRC in carriers of the MTR2756G allele was 2 times higher than that of the wild-type homozygote (AA) (95% CI 1.22 to 3.40). Compared with those who were both MTHFR1298AA and MTR2756AA genotypes, those who were both MTHFRl298AA and MTR2756AG/GG genotypes had a significantly higher risk of CRC (OR=2.57, 95% CI 1.42 to 4.65). There was a joint effect between the two (P=0.04). Conclusion The MTR2756G allele may be a risk factor for CRC, and there is a joint effect between MTHFRA1298C and MTRA2756G.