论文部分内容阅读
采用16S rDNA特征序列PCR-DGGE法,分析了不同饵料饲养的暗纹东方鲀的表皮、肠道和河鲀毒素累积组织(肝和卵巢)中可培养细菌的群落组成。根据分子系统发育分析,共鉴定出45种可培养细菌,在这些细菌中,以变形细菌的gamm a亚群占多数,其它分别隶属于低GC含量革兰氏阳性菌和高GC含量革兰氏阳性菌。摄食不同饵料的暗纹东方鲀,其肠道、表皮或河鲀毒素累积组织中的细菌菌落组成是不同的,但不管是摄食人工配合饲料或天然饵料,在暗纹东方鲀的肠道、表皮或河鲀毒素累积组织中,均发现已有报道的可产河鲀毒素的细菌类群,表明饵料来源对暗纹东方鲀的河鲀毒素产生不是必需的。
Using 16S rDNA sequence PCR-DGGE method, the community composition of culturable bacteria in the epidermis, intestinal tract and tetrodotoxin accumulating tissues (liver and ovary) of Takifugu obscurus fed on different diets was analyzed. According to molecular phylogenetic analysis, a total of 45 kinds of culturable bacteria were identified. Of these bacteria, the majority of gamm a subgroups of the bacteria were classified, and the other strains belonged to Gram-positive bacteria with low GC content and Gram Positive bacteria. The composition of bacterial colonies in the intestine, epidermis or tetrodotoxin accumulation tissues is different for feeding on different dietary members of the obtuse-flowering Takifugu fasciatus, but regardless of the ingestion of artificial diet or natural feed, Or tetrodotoxin accumulating tissues, it has been found that there are reported bacterial isolates of tetrodotoxin, indicating that the source of feed is not necessary for the production of tetrodotoxin in Ushmattes.