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应用套式ＰＣＲ检测了８８例丙型肝炎患者血清和外周血单个核细胞（ＰＢＭＣ）中ＨＣＶＲＮＡ；结果发现：慢性丙肝患者ＰＢＭＣ中ＨＣＶＲＮＡ检出率显著高于急性丙肝患者（Ｐ＜０．００１）；急、慢性丙肝患者血清和慢性丙肝患者ＰＢＭＣ中ＨＣＶＲＮＡ检出率显著高于ＡＬＴ正常的抗－ＨＣＶ阳性者（Ｐ＜０．００１）；少数患者血清中ＨＣＶＲＮＡ阴性，ＰＢＭＣ中可测及，部分患者血清抗一ＨＣＶ阴性，而血清ＨＣＶＲＮＡ阳性．提示：血清ＨＣＶＲＮＡ检测有助于抗－ＨＣＶ阴性丙肝的诊断和早期诊断；丙肝的肝损害可能与ＨＣＶＲＮＡ血症有关；ＰＢＭＣ中ＨＣＶ感染在丙肝的慢性化和慢性肝损害中可能起一定作用；ＰＢＭＣ中可贮存ＨＣＶＲＮＡ。
The detection of HCVRNA in serum and peripheral blood mononuclear cells (PBMCs) of 88 patients with hepatitis C by nested PCR showed that the detection rate of HCV RNA in PBMC of patients with chronic hepatitis C was significantly higher than that of patients with acute hepatitis C (P <0.001) ; The detection rate of HCV RNA in PBMC of acute and chronic hepatitis C patients and chronic hepatitis C patients was significantly higher than that of normal ALT positive anti-HCV positive patients (P <0.001); few patients serum HCVRNA negative PBMC measurable and part Patient serum anti-HCV negative, and serum HCV RNA positive. It is suggested that the detection of serum HCVRNA contributes to the diagnosis and early diagnosis of anti-HCV negative hepatitis C. The liver damage of hepatitis C may be related to HCV RNA. HCV infection in PBMC may play a role in chronic hepatitis C and chronic liver damage. PBMC In which HCVRNA can be stored.