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以Gunnera/Nostoc共生体为材料,研究、比较了碳水化合物和H2对其固氮活力及呼吸速率的影响,结果表明,在正常条件下,外源H2可提高Gunnera/Nostoc共生体固氮活力近100%,却使其呼吸速率下降近50%;而碳水化合物(葡萄糖)也可使其固氮活力提高约100%,并使其呼吸速率提高近160%。表明碳水化合物及H2均可为Gunnera/Nostoc共生体提供固氮过程的还原力。由于H2的循环利用降低了呼吸速率,所以就间接地节约了Gunnera/Nostoc共生体固氮过程中对碳水化合物的消耗。结果还表明,碳水化合物(葡萄糖)对恢复饥饿后的Gunnera/Nostoc共生体固氮活力的作用优于外源H2。
Using Gunnera / Nostoc symbionts as material, the effects of carbohydrates and H2 on its nitrogen fixation activity and respiration rate were studied. Exogenous H2 increased the nitrogen fixation activity of Gunnera / Nostoc symbiont nearly 100% under normal conditions, , But decreased its respiration rate by nearly 50%. Carbohydrate (glucose) also increased its nitrogen fixation activity by about 100% and increased its respiration rate by nearly 160%. It shows that carbohydrates and H2 can all provide the reducing power of Gunnera / Nostoc symbiont to fix nitrogen. As the recycle of H2 reduces the respiration rate, the carbohydrate consumption of Gunnera / Nostoc symbionts during nitrogen fixation is indirectly saved. The results also showed that the effect of carbohydrates (glucose) on the nitrogen fixation activity of Gunnera / Nostoc symbionts after starvation was better than that of exogenous H2.