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目的 构建大鼠反义p3 8αMAPK腺病毒载体。方法 用RT PCR法扩增大鼠p3 8αcDNA片段 ,连接到T载体进行测序 ,经测序正确后与pAdtrack cmvvector重组 ,最后转入AdEasy(tm)XLAdenoviralVectorSystem系统中 ,得到p3 8α基因腺病毒载体。结果 成功构建大鼠p3 8α腺病毒载体。结论 为在基因水平研究p3 8α的作用提供了良好工具。
Objective To construct rat antisense p3 8αMAPK adenovirus vector. Methods The rat p3 8α cDNA fragment was amplified by RT-PCR and ligated to T vector for sequencing. After sequencing, the recombinant plasmid was recombined with pAdtrack cmvvector and finally transferred into AdEasy (TM) XLAdenoviral Vector System to obtain p3a gene adenovirus vector. Results The rat p3 8α adenovirus vector was successfully constructed. Conclusion This provides a good tool for studying the role of p3 8α at the genetic level.