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目的:研究清肝灵煎剂(清肝灵)对急性肝损伤大鼠内毒素血症的治疗作用。方法:SD大鼠48只,随机分为正常对照组、模型组、茵栀黄注射液治疗组、清肝灵预保护组、清肝灵低剂量组以及清肝灵高剂量组。除正常对照组外,其它5组均用ANIT(100mg/kg体重)灌胃以制备大鼠急性肝损伤模型。清肝灵预保护组于ANIT攻毒前3天起给药,所有实验组均于ANIT攻毒后分别给予相应药物治疗。ANIT攻毒后72h,检测各组大鼠的血清ALT、AST和TBil水平、、内毒素(ET)、一氧化氮(NO)与肿瘤坏死因子(TNF-α)水平以及肝组织病理的变化。结果:与模型组比较,各清肝灵治疗组的血清ALT、AST及TBil均明显下降,且肝组织病变程度明显减轻;各清肝灵治疗组大鼠血清ET、TNF-α和NO水平均明显降低。结论:清肝灵能减轻肝损伤大鼠的内毒素血症,此可能是其防治大鼠肝损伤的作用机制之一。
Objective: To study the therapeutic effect of Qingganling decoction (Qingganling) on endotoxemia in acute liver injury rats. Methods: Forty-eight SD rats were randomly divided into normal control group, model group, Yinzhihuang injection treatment group, Qingganling pre-protection group, Qingganling low dose group and Qingganling high dose group. Except normal control group, the other five groups were fed with ANIT (100mg / kg body weight) to prepare acute liver injury model in rats. Qingganling pre-protection group was administered 3 days prior to ANIT challenge, and all the experimental groups were given appropriate drug treatment after ANIT challenge. The levels of serum ALT, AST and TBil, the levels of endotoxin (ET), nitric oxide (NO) and tumor necrosis factor (TNF-α) and the changes of liver histopathology were detected 72 h after ANIT challenge. Results: Compared with the model group, the serum ALT, AST and TBil in each group were significantly decreased, and the degree of liver lesions was significantly reduced. The levels of ET, TNF-α and NO in serum of Qingqiling group were significantly lower than those in model group Obvious reduction. Conclusion: Qinggan Ling can reduce endotoxemia in rats with liver injury, which may be one of the mechanisms of prevention and treatment of hepatic injury in rats.