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目的:观察止痛祛风汤对急性痛风性关节炎大鼠血清白细胞介素(IL)-6、IL-8的影响。方法:将60只健康雄性Wistar大鼠随机分为空白对照组,模型对照组,秋水仙碱组,止痛祛风汤高、中、低剂量组,每组10只。空白对照组大鼠不限制饮水,自由进食;模型对照组大鼠给予生理盐水0.135 mg·kg~(-1)进行灌胃;秋水仙碱组大鼠给予秋水仙碱溶液0.135 mg·kg~(-1)进行灌胃;止痛祛风汤高、中、低剂量组大鼠分别给予止痛祛风汤31.25 g·(100 g)~(-1)、15 g·(100 g)~(-1)、7.5 g·(100 g)~(-1)进行灌胃。于第7天给药后30 min,除空白对照组外,其余各组大鼠采用注射尿酸钠方式诱导急性痛风性关节炎大鼠模型,分别检测各组大鼠血清IL-6、IL-8水平的变化情况。结果:止痛祛风汤高、中剂量组以及秋水仙碱组大鼠血清中的IL-6、IL-8含量均明显降低,3组分别与模型对照组进行比较,差异均有统计学意义(P<0.05);止痛祛风汤高、中剂量组与秋水仙碱组比较,差异无统计学意义(P>0.05)。结论:止痛祛风汤能通过降低IL-6、IL-8的水平而达到治疗痛风性关节炎的作用。
Objective: To observe the effect of Zhitong Qufeng Decoction on serum interleukin (IL) -6 and IL-8 in rats with acute gouty arthritis. Methods: Sixty healthy male Wistar rats were randomly divided into blank control group, model control group, colchicine group, and Zhitong Qufeng Decoction high, medium and low dose group, with 10 rats in each group. Rats in the blank control group were fed with water without restriction. Rats in the model control group were administered with saline 0.135 mg · kg -1 (-1) orally. Rats in the colchicine group were treated with colchicine 0.135 mg · kg -1 -1). The rats in the high, middle and low doses of Zhitong Qufeng Decoction were given 31 g · (100 g) -1, 15 g · (100 g) -1 ), 7.5 g · (100 g) -1. At 30 days after administration on day 7, except for the blank control group, the rats in each group were injected with sodium urate to induce acute gouty arthritis rat models. The levels of IL-6, IL-8 The level of change. Results: The levels of IL-6 and IL-8 in the serum of the high-dose, middle-dose and colchicine groups were significantly lower than those of the model group. The differences were statistically significant (P <0.05) P <0.05); analgesia Qufeng Decoction high and medium dose group and colchicine group, the difference was not statistically significant (P> 0.05). Conclusion: Analgesic Qufeng Decoction can reduce the levels of IL-6, IL-8 to achieve the treatment of gouty arthritis.