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目的:观察黄连总碱对结肠癌的化学预防作用。方法:以二甲肼(1-2 dimethylhydrazine,DMH)40 mg·g-1 sc+1%葡聚糖硫酸钠(dextran sodium sulfate,DSS)水溶液饮用诱导形成大鼠结肠癌模型。观察黄连总碱(120 mg·g-1·d-1,1周5d)和小檗碱(100 mg·g-1·d-1,1周5 d)ig,连续16周。观察对大鼠体重、异常隐窝灶(abrrant crypt foci,ACF)和结肠癌发生率的影响。结果:与模型组相比,黄连总碱明显改善DMH+DSS诱癌过程中大鼠的恶液质状态并阻遏体重减轻,显著减少大鼠结肠ACF数和明显降低结肠癌的发生率;其作用与小檗碱相似。结论:黄连总碱能抑制DMH和DSS联合使用诱导大鼠早期ACF的形成和结肠癌发生,该作用可能与其主要成分小檗碱有关。
Objective: To observe the chemical preventive effect of berberine on colon cancer. Methods: The rat colon cancer model was induced by drinking with 40 mg · g -1 sc + 1% dextran sodium sulfate (DSS) solution with 1-2 dimethylhydrazine (DMH). The berberine (100 mg · g-1 · d-1, 1 week, 5 d) ig was observed for 6 weeks in the rhizoma coptidis (120 mg · g-1 · d-1,1 weeks). The effects on the body weight, abrrant crypt foci (ACF) and the incidence of colon cancer in rats were observed. Results: Compared with model group, Coptidis Rhizoma alkaloids significantly ameliorate catecholamines and repress weight loss in rats induced by DMH + DSS, significantly reduced colon ACF number and significantly decreased the incidence of colon cancer; Similar to berberine. Conclusion: Coptis alkaloids can inhibit the combination of DMH and DSS induced early ACF formation and colon cancer, the effect may be related to its main component of berberine.