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目的探讨前列腺素E1对睾丸生精功能保护作用。方法建立大鼠睾丸扭转损伤的病理模型。45只大鼠随机分成3组,每组15只。A组为假手术对照组;B组大鼠睾丸扭转720度,维持2h复位,并用前列腺素E145μg·kg-1.d-1治疗;C组损伤同B组,但不予治疗。所有大鼠在手术后40d处死,取睾丸组织,肉眼观察其形态,测量长度,称其重量,计算脏器系数,行常规HE染色后镜下观察睾丸的病理改变。结果 B组睾丸长度明显长于C组[(1.99±0.07)cmvs.(1.79±0.21)cm],C组睾丸长度明显短于A组[(1.79±0.21)cmvs.(2.16±0.12)cm](P<0.05)。镜下观察:B组损伤明显轻于C组。脏器系数:B组与A组相仿,但明显大于C组[(11.98±0.89)vs.(7.86±1.07)](P<0.05)。结论前列腺素E对睾丸生精功能有明显保护作用。
Objective To investigate the protective effect of prostaglandin E1 on testicular spermatogenesis. Methods The pathological model of testicular torsion injury in rats was established. Forty-five rats were randomly divided into three groups of 15 rats. Group A was sham operation control group; Group B rats testicular torsion 720 degrees, maintained 2h reset, and prostaglandin E145μg · kg-1.d-1 treatment; C group with the same injury B, but not treated. All rats were killed 40 days after operation, testicular tissue was taken, the morphology was observed with the naked eye, the length was measured, the weight was measured, the organ coefficient was calculated, and pathological changes of testis were observed under conventional HE staining. Results The length of testis in group B was significantly longer than that in group C [(1.99 ± 0.07) cm vs 1.79 ± 0.21 cm]. The length of testis in group C was significantly shorter than that in group A [(1.79 ± 0.21) cm vs (2.16 ± 0.12) cm] P <0.05). Microscopic observation: B group was significantly lighter than C group. Organ coefficient: Group B was similar to group A, but significantly larger than group C [(11.98 ± 0.89) vs. (7.86 ± 1.07)] (P <0.05). Conclusion Prostaglandin E has a significant protective effect on testicular spermatogenesis.