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目的 充分利用酸性成纤维细胞生长因子（ａＦＧＦ） 促进造血重建的作用，以进一步完善程序移植新方法。方法 以昆明种小鼠急性放射病为模型，进行了骨髓细胞（ＢＭＣ） 经自牛脑中制备的ａＦＧＦ孵育后程序移植对造血重建、急性移植物抗宿生病（ＧＶＨＤ） 的研究。结果 ａＦＧＦ 孵育后程序移植ＢＭＣ４ ×１０６ ，存活率达４０％ ，比单纯一次移植ＢＭＣ１ ×１０７ 组高（３０％ ），但不如单纯骨髓程序移植ＢＭＣ４ ×１０６ 组（６０％ ）；另一方面，外周血白细胞、骨髓有核细胞计数、ＣＦＵＥ、ＣＦＵＧＭ、ＣＦＵＳ比一次骨髓移植１×１０７ 组和单纯骨髓程序移植ＢＭＣ４ ×１０６ 回升快，而ＣＦＵＦ回升无差别；ＧＶＨＤ较一次骨髓移植１ ×１０７ 组轻，较骨髓程序移植４ ×１０６ 重。结论 引起ＧＶＨＤ 的主要原因是异基因Ｔ淋巴细胞，通过ａＦＧＦ孵育后程序移植可以更充分地发挥其促进造血重建的作用，而减轻ＧＶＨＤ。
Objective To fully utilize the role of acidic fibroblast growth factor (aFGF) in promoting hematopoietic reconstitution to further improve the new method of procedural transplantation. Methods The model of acute radiation sickness in Kunming mice was used to study the hematopoietic reconstitution and acute graft-versus-host-disease (GVHD) after transplantation of bone marrow cells (BMCs) via aFGF prepared from bovine brain. Results After aFGF incubation, BMC4 × 106 cells were transplanted into the BMC4 × 106 group (40%), which was 40% higher than that of the BMC1 × 107 group (30%). However, the peripheral blood mononuclear cells White blood cells, bone marrow nucleated cells count, CFU E, CFU MG, CFU S than a bone marrow transplantation 1 × 107 group and simple bone marrow transplantation BMC4 × 106 rebound faster, while CFU F no difference in recovery; GVHD more than once Bone marrow transplantation 1 × 107 group of light, compared with bone marrow transplantation 4 × 106 weight. Conclusions The main cause of GVHD is allogeneic T lymphocytes. After aFGF transplantation, the transplantation of aFGF can fully exert its effect of promoting hematopoietic reconstitution and reduce GVHD.