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建立了同时测定玫瑰花口服液中没食子酸、芦丁、槲皮素和山奈素的高效液相色谱(HPLC)测定方法。采用Agilent HC-C18色谱柱(250×4.6mm,5μm);流动相为0.5%磷酸-甲醇,梯度洗脱;流速0.8mL·min-1;柱温30℃;检测波长260nm。结果显示:没食子酸在40.7～244.20μg·mL-1、芦丁在25～150μg·mL-1、槲皮素在13.3～80μg·mL-1、山奈素在2.69～16.2μg·mL-1线性关系良好,相关系数分别为0.9997、0.9993、0.9998、0.9994。4组分平均回收率分别为99.2%、101.2%、98.8%、99.2%,相对标准偏差(RSD)分别为0.60%、1.02%、0.62%、0.57%(n=3)。该方法准确、简便,适合上述4种有效成分的同时测定。
A high performance liquid chromatography (HPLC) method was established for simultaneous determination of gallic acid, rutin, quercetin and kaempferol in Rose Oral Liquid. The mobile phase was 0.5% phosphoric acid-methanol, gradient elution was performed on an Agilent HC-C18 column (250 × 4.6 mm, 5 μm). The flow rate was 0.8 mL · min-1. The results showed that the linear range of the content of gallic acid was 40.7-244.20μg · mL-1, rutin was 25-150μg · mL-1, quercetin was 13.3-80μg · mL-1, and kaempferol was 2.69-16.2μg · mL-1 The correlation coefficients were 0.9997, 0.9993, 0.9998 and 0.9994 respectively. The average recoveries were 99.2%, 101.2%, 98.8% and 99.2%, respectively. The relative standard deviations (RSDs) were 0.60%, 1.02% and 0.62 %, 0.57% (n = 3). The method is accurate and simple, suitable for the simultaneous determination of the above four active ingredients.