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我们曾报道McAb-HSA-MTX的制备及其体外选择性细胞毒作用的实验研究,为进一步研究交联物在体内的稳定性,首先我们建立了人前B细胞(Nalm-6)白血病小鼠模型:给未经照射的BALB/c裸鼠直接接种Nalm-6细胞后无一生长肿瘤。以γ射线对BALB/c裸鼠全身照射,每7天1次,连续照射3次,每次剂量200 Gy,末次照射后第三天接种Nalm-6细胞亦未出现移植瘤。而接受相同剂量照射的裸鼠,首次照射后1周进行第二次照射,间隔3天再第三次照射,末次照射后第三天接种Nalm-6细胞,接种后7～17天,9只裸鼠中有8只出现移植瘤,经硷性磷酶桥联酶标显色技术及间接免疫荧光法鉴定,移植瘤确系Nalm-6细胞。
We have reported the preparation of McAb-HSA-MTX and its selective cytotoxicity in vitro. To further investigate the stability of cross-linked compounds in vivo, we first established a mouse model of human pre-B cell (Nalm-6) leukemia. : None of the tumors grew after directly inoculating Nalm-6 cells in non-irradiated BALB/c nude mice. The whole body of BALB/c nude mice was irradiated with γ-rays once every 7 days for 3 consecutive times with a dose of 200 Gy. Nalm-6 cells were inoculated on the third day after the last irradiation. The nude mice that received the same dose were irradiated for the second time one week after the first irradiation, three days after the third irradiation, and Nalm-6 cells were inoculated on the third day after the last irradiation, and 7 to 17 days after inoculation, 9 Eight transplanted tumors were found in nude mice, identified by sputum phosphatase bridging enzyme labeling and indirect immunofluorescence, and the transplanted tumors were indeed Nalm-6 cells.