We investigate the DO-D-O mixing through the doubly Cabibbo suppressed (DCS) channel DO→fO(980)K*O and its charge conjugate channel,in which the K*O meson is reconstructed in both K+π- and KsπO final state.Although the decay DO→ fo(980)K* has a small branching ratio,the final state mesons are relatively easy to identify.The fo(980) meson can be replaced by the S-wave π+π- state,or a longitudinally polarized vector meson pO.All mixing parameters,including the mass difference and decay width difference,can be extracted by studying the time-dependent decay width of these channels.We show that the method is valid in all regions for mixing parameters and it does not depend on the strong phase difference.