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目的:了解脂质过氧化对实验性肝纤维化的影响。方法:将36只雄性Wistar大鼠分为正常组(N组)、3、6、9周组(A、B、C组),用CCL_4及5％酒精诱导肝纤维化模型,分别在3、6、9周宰杀动物,观察三组肝组织病理改变及检测肝组织中丙二醛(MDA)和羟脯氨酸(HYP)含量变化。结果:A组肝组织中MDA和HYP含量明显高于正常大鼠组(P<0.01)。随染毒时间延长,肝组织中MDA和HYP含量不断增加,三组间有显著性差异(P<0.01)。光镜下,A、B、C组肝组织呈现不同程度的肝纤维化,染毒时间越长纤维化程度越重。肝组织中HYP含量变化与MDA含量变化呈正相关,γ为0.80(P<0.01)。结论:CCL_4及低浓度酒精诱导的肝纤维化与肝脂质过氧化有关。
Objective: To understand the effects of lipid peroxidation on experimental liver fibrosis. Methods: Thirty-six male Wistar rats were divided into normal group (N group), 3, 6 and 9 weeks group (A, B and C). Liver fibrosis models were induced by CCL_4 and 5% The animals were slaughtered at 6 and 9 weeks. The pathological changes of the liver tissues and the content of malondialdehyde (MDA) and hydroxyproline (HYP) in the liver tissues were observed. Results: The contents of MDA and HYP in group A were significantly higher than those in normal group (P <0.01). With the prolongation of exposure time, the content of MDA and HYP in liver tissue increased continuously, with significant difference among the three groups (P <0.01). Light microscope, A, B, C group of liver tissue showed varying degrees of liver fibrosis, exposure to the longer the more severe fibrosis. The change of HYP content in liver tissue was positively correlated with the change of MDA content, γ was 0.80 (P <0.01). Conclusion: Liver fibrosis induced by CCL_4 and low alcohol concentration is related to hepatic lipid peroxidation.