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目的:探讨细胞外信号调节激酶(ERK)及其磷酸化状态(p-ERK)在晚期前列腺癌(PCa)组织中的表达,判断其能否作为晚期前列腺癌预后的标志物。方法:应用免疫组化(Envision)法检测20例BPH组织及40例PCa组织中ERK1/2及其p-ERK1/2的表达,并分析其与PCa转移(侵袭力)、Gleason评分、PSA水平以及预后的关系。结果:(1)ERK1/2的表达:BPH中表达率为55.0%,PCa中表达率为82.5%,有显著性差异(P<0.05);与晚期PCa侵袭力、Gleason评分、PSA水平、生存时间无关联(P>0.05);(2)p-ERK1/2的表达:BPH中表达率为35.0%,PCa中表达率为75.0%,有显著性差异(P<0.05);而与Gleason评分、PSA水平无关联(P>0.05);在转移和未转移组、生存时间>5年与生存时间≤5年组中阳性表达率分别为:61.9%、89.5%、57.9%、90.5%,有显著性差异(P<0.05)。结论:ERK1/2、p-ERK1/2在晚期前列腺癌组织中高表达,ERK磷酸化与晚期PCa侵袭力及预后有关。
Objective: To investigate the expression of extracellular signal-regulated kinase (ERK) and its phosphorylation status (p-ERK) in advanced prostate cancer (PCa) tissues and to determine whether it can be used as a marker of the prognosis of advanced prostate cancer. Methods: The expressions of ERK1 / 2 and p-ERK1 / 2 in 20 cases of BPH and 40 cases of PCa were detected by immunohistochemistry. The expressions of ERK1 / 2 and p-ERK1 / 2 in PCa tissues were compared with those of PCa (invasiveness), Gleason score And the relationship between the prognosis. Results: (1) The expression of ERK1 / 2 was significantly higher in BPH than that in BPH (55.0%, PCa, 82.5%, P <0.05) (P> 0.05). (2) The expression of p-ERK1 / 2 was significantly higher in BPH than that in BPH (35.0% vs 75.0%, P <0.05) (P> 0.05). The positive rates of survival in the metastasis group and the non-metastasis group were 61.9%, 89.5%, 57.9% and 90.5%, respectively Significant difference (P <0.05). Conclusion: ERK1 / 2 and p-ERK1 / 2 are highly expressed in advanced prostate cancer. The phosphorylation of ERK is related to the invasiveness and prognosis of advanced PCa.