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目的 观察自体骨骼肌干细胞移植于缺血心肌后对心肌纤维化的抑制作用。方法 取12只成年犬臀大肌 ,分离卫星细胞、培养、传代 ,用 4′,6 二乙酰基 2 苯基吲哚 (DAPI)标记卫星细胞 ;在已建立的犬急性心肌梗死动物模型基础上 ;将DAPI标记的卫星细胞 ,自左冠状动脉前降支灌入缺血心肌中。分别于 2、4、8周后取出心脏 ,对缺血心肌的纤维化程度及植入的卫星细胞进行观察 ,并设对照组。结果 卫星细胞在缺血心肌中可分化成心肌细胞样细胞 ;在卫星细胞移植区域 ,心肌细胞的纤维化被有效抑制 ;对照组缺血心肌发生玻璃样变性 ,心肌细胞基本结构紊乱。结论 自体骨骼肌卫星细胞在缺血心肌中可分化成心肌细胞样细胞 ,并可抑制缺血心肌的纤维化 ;骨骼肌干细胞移植有望为心肌损伤提供一条新的治疗途径。
Objective To observe the inhibitory effect of autologous skeletal muscle stem cells on myocardial fibrosis after transplantation into ischemic myocardium. Methods The adult male canine gluteus maximus was isolated from 12 adult dogs. The satellite cells were isolated and cultured. The satellite cells were labeled with 4 ’, 6 diacetyl 2 phenylindole (DAPI). On the basis of established animal model of acute myocardial infarction DAPI-labeled satellite cells were infused into the ischemic myocardium from the anterior descending branch of the left coronary artery. After 2, 4, and 8 weeks respectively, the heart was taken out, the degree of fibrosis of ischemic myocardium and satellite cells implanted were observed, and the control group was set up. Results Satellite cells differentiated into cardiomyocyte-like cells in ischemic myocardium. In the satellite cell transplantation area, fibrosis of cardiomyocytes was effectively inhibited. In the control group, the ischemic myocardium was vitrified and the cardiomyocytes were disorganized. Conclusion Autologous skeletal muscle satellite cells can differentiate into cardiomyocyte-like cells in ischemic myocardium and inhibit the fibrosis of ischemic myocardium. Skeletal muscle stem cell transplantation is expected to provide a new therapeutic approach for myocardial injury.