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目的：ＳＥＢ诱导增殖的外周血Ｔ细胞是否具有正常Ｔ细胞的功能。方法：用超抗原刺激淋巴细胞生长；用流式细胞测定技术检查细胞表面ＣＤ４，ＣＤ８，ＣＤ２８，ＴＣＤ１６的抗原表达量；加入同源，异源抗原检查自身反应性。结果：ＳＥＢ能诱导外周血ＣＤ４＋Ｔ细胞的增殖。随ＣＤ４＋Ｔ细胞的增加，ＣＤ２８分子表达上升。外周增殖的ＣＤ４＋Ｔ细胞与胸腺内分化的Ｔ细胞相同，９７％以上的ＣＤ４＋Ｔ细胞都表达ＣＤ２８分子。但对ＣＤ１６阳性的抗原提呈细胞的依赖性下降，并显示对自身抗原的识别能力增强。结论：外周活化的ＣＤ４＋Ｔ细胞与胸腺内分化的ＣＤ４＋Ｔ细胞功能不同。
OBJECTIVE: To investigate whether the proliferation of peripheral blood T cells induced by SEB has the function of normal T cells. Methods: The superantigens were used to stimulate the growth of lymphocytes; the expression of CD4, CD8, CD28 and TCD16 on the surface of cells was detected by flow cytometry; autologous reactions were detected by adding homologous and heterologous antigens. Results: SEB can induce the proliferation of CD4 + T cells in peripheral blood. With the increase of CD4 + T cells, the expression of CD28 molecules increased. Peripherally proliferating CD4 + T cells are identical to differentiated T cells in the thymus, with more than 97% of CD4 + T cells expressing CD28 molecules. But decreased its dependence on CD16-positive antigen-presenting cells and showed an increased ability to recognize autoantigens. CONCLUSION: Peripherally activated CD4 + T cells function differently than differentiated CD4 + T cells in the thymus.