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【目的】比较SSR和SCoT分子标记在美洲黑杨×青杨派杂种无性系遗传差异性分析上的应用性。【方法】利用SSR和SCoT标记,对9个美洲黑杨×青杨派杂种无性系及3个亲本的遗传差异性进行分析。【结果】筛选出的12对SSR引物对供试杨树材料共扩增出94条清晰条带,其中多态性条带85条,多态性比率达到90%,标记指数为3.19;筛选出的14条SCoT引物对供试杨树材料共扩增出清晰条带127条,其中多态性条带95条,多态性比率达到75%,标记指数为2.72。聚类分析表明,SSR和SCoT分子标记得出12个无性系的遗传相似系数分别为0.44~0.80和0.40~0.87,平均遗传相似系数分别为0.62和0.64;在相似系数平均值处,SSR和SCoT分子标记将12个杨树材料分别分为3大类和5大类。Mantel检测显示,2种分子标记在12个无性系间的遗传相似性显著相关(r=0.501 3,P=0.003)。【结论】这2种分子标记均适合美洲黑杨×青杨派杂种无性系鉴定和遗传多样性研究,但SSR标记的多态性比率和标记效率均高于SCoT标记。
【Objective】 The objective of this study was to compare the applicability of SSR markers and SCoT markers in genetic diversity analysis of hybrid Populus × Populus × black poplar clones. 【Method】 The genetic diversity of nine Populus × Populus × Populus hybridis clones and three parents were analyzed by SSR and SCoT markers. 【Result】 A total of 94 clear bands were amplified from 12 pairs of SSR primer pairs. Among them, 85 bands were polymorphic, with a polymorphism rate of 90% and a marker index of 3.19. Screening out A total of 127 clear bands were amplified from 14 SCoT primer pairs. Among them, 95 were polymorphic bands with a polymorphism rate of 75% and a marker index of 2.72. Cluster analysis showed that the genetic similarity coefficients of 12 clones were 0.44 ~ 0.80 and 0.40 ~ 0.87 respectively with SSR and SCoT markers, and the average genetic similarity coefficients were 0.62 and 0.64, respectively. At the mean of similarity coefficient, SSR and SCoT Molecular marker 12 poplar materials were divided into 3 categories and 5 categories. The Mantel test showed that there was a significant correlation between the genetic similarity of the two molecular markers in 12 clones (r = 0.501 3, P = 0.003). 【Conclusion】 Both of these two molecular markers are suitable for clonal identification and genetic diversity of hybrid Populus × Populus × Populus × black poplar. However, the polymorphism rate and labeling efficiency of SSR markers were higher than those of SCoT markers.