论文部分内容阅读
通过表面引发原子转移自由基聚合(ATRP)在硅表面接枝了聚(N-异丙基丙烯酰胺)(PNIPAAm)聚合物刷,并考察了PNIPAAm改性表面在单一蛋白质溶液以及血浆中与血浆蛋白质之间的相互作用.蛋白质吸附测试表明,与未改性的硅表面相比,改性后的表面对纤维蛋白原的吸附量大大降低,特别是在血浆中纤维蛋白原吸附量小于5 ng/cm2.不同血浆浓度下的蛋白质吸附测试发现PNIPAAm改性后的表面并没有出现明显的“Vroman”效应,说明改性后的表面在与血液接触的初期具有良好的阻抗纤维蛋白原吸附的能力.此外,蛋白质免疫印迹测试进一步发现PNIPAAm改性表面能够排斥绝大多数血浆蛋白质,特别是在凝血过程中起重要作用的几种蛋白质的吸附.实验结果表明,PNIPAAm作为一种常见的温敏性聚合物,同时也具有了良好的排斥纤维蛋白原的非特异性吸附能力和较好的血液相容性.
Poly (N-isopropylacrylamide) (PNIPAAm) polymer brushes were grafted onto the silicon surface by surface-initiated atom transfer radical polymerization (ATRP) and investigated the effect of modified PNIPAAm modified surfaces on single protein solution and plasma and plasma Protein Interactions Protein adsorption tests showed that the adsorbed amount of fibrinogen on the modified surface is greatly reduced compared to the unmodified silicon surface, especially when the amount of fibrinogen adsorbed in the plasma is less than 5 ng / cm2. The protein adsorption test at different plasma concentrations showed that there was no obvious “Vroman ” effect on the modified surface of PNIPAAm, indicating that the modified surface had good impedance to fibrin adsorption In addition, Western blotting analysis further revealed that PNIPAAm modified surface was able to repel the adsorption of most plasma proteins, especially several proteins that play an important role in the process of blood coagulation.The experimental results showed that PNIPAAm as a common temperature Sensitive polymers, but also has a good rejection of fibrinogen non-specific adsorption capacity and better blood compatibility.