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目的:建立HPLC同时测定温胆汤中甘草苷、柚皮苷、橙皮苷和甘草酸含量的方法。方法:DIKMA Diamonsil(2)-C18柱(4.6 mm×250 mm,5μm);流动相乙腈(A)-0.1%磷酸溶液(B),线性梯度洗脱;检测波长237,283 nm;柱温25℃;流速1.0 mL.min-1;进样量10μL。结果:甘草苷、柚皮苷、橙皮苷和甘草酸铵的进样量与峰面积,分别在0.019 9~0.119(r=0.999 7),0.180~1.08(r=0.999 7),0.146~0.873(r=0.999 8),0.0393~0.236μg(r=0.999 7)呈良好的线性关系;平均加样回收率依次为97.7%,97.7%,97.1%,98.5%,RSD 1.4%,2.0%,2.0%,1.9%。结论:该方法快速,简便,重复性好,适合于同时测定温胆汤样品中甘草苷、柚皮苷、橙皮苷和甘草酸的含量。
Objective: To establish a method for the simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizin in Wendan Decoction by HPLC. The mobile phase consisted of acetonitrile (A) and 0.1% phosphoric acid solution (B) with linear gradient elution. The detection wavelength was 237 and 283 nm. The column temperature was 25 ℃. Flow rate 1.0 mL.min-1; injection volume 10μL. Results: The injection volume and peak area of liquiritin, naringin, hesperidin and ammonium glycyrrhizinate were between 0.0199 and 0.119 (r = 0.999 7), 0.180-1.08 (r = 0.999 7) and 0.146-0.873 (r = 0.999 8) and 0.0393-0.236μg (r = 0.999 7). The average recoveries were 97.7%, 97.7%, 97.1%, 98.5%, RSD 1.4%, 2.0%, 2.0 %, 1.9%. Conclusion: The method is rapid, simple and reproducible. It is suitable for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizin in Wen Dan Tang samples.