论文部分内容阅读
本文观察了小鼠肺腺癌ＬＡ７９５母系及不同转移潜能克隆株ＬＡ１、ＬＡ５细胞其胞内［Ｃａ￣（２＋）］及诱导分化剂维胺酸和二甲基亚砜对其影响。发现母系与克隆株细胞间，其胞内静息［Ｃａ￣（２＋）］确有差异（Ｐ＜０．０１）。中转移ＬＡ５克隆株胞内［Ｃａ￣（２＋）］最高，不转移ＬＡ１克隆株最低，母系ＬＡ７０５胞内［Ｃａ￣（２＋）］居中。在克隆株细胞中，胞内［Ｃａ￣（２＋）］与其转移潜能成正比。维胺酸作用不同时间，ＬＡ７９５、ＬＡ１、ＬＡ５胞内［Ｃａ￣（２＋）］先下降，然后逐渐恢复用药前水平，最终至胞内［Ｃａ￣（２＋）］升高。二甲基亚砜作用后，引起胞内［Ｃａ￣（２＋）］一过性升高，然后下降最后又升高。二者均终至所用细胞内［Ｃａ￣（２＋）］升高，提示其诱导分化作用过程中，［Ｃａ￣（２＋）］变化起作用，胞内［Ｃａ￣（２＋）］与肿瘤转移性有一定关系。
In this study, we investigated the effects of levofloxacin and dimethyl sulfoxide (DMSO) on the intracellular [Ca ~ (2+)] and the differentiation of mouse LA795 breast cancer cells and different metastatic potential clones LA1 and LA5. It was found that there was a significant difference in intracellular resting [Ca ~ (2+)] between maternal and cloned cells (P <0.01). LA5 clones had the highest intracellular [Ca ~ (2+)], the lowest LA1 clones without metastasis, and the intracellular [Ca ~ (2+)] in maternal LA705. Intracellular [Ca ~ (2+)] is directly proportional to its metastatic potential in clonal cells. At different time points, the intracellular [Ca ~ (2+)] of LA795, LA1 and LA5 decreased first and then gradually recovered to the level of pretreatment, finally the intracellular [Ca ~ (2+)] increased. Dimethyl sulfoxide, caused intracellular [Ca ~ (2+)] transiently increased, and then decreased and finally increased. Both of them eventually reach the level of [Ca 2+] in the cells used, which suggests that [Ca 2+] plays a role in the process of differentiation induction. Intracellular [Ca 2+] and tumor metastasis Have a certain relationship.