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目的:探讨胃癌患者外周血单个核细胞(PBMC)中Th1和Th2类细胞因子mRNA表达变化及其临床意义。方法:采用RT-PCR技术,检测102例胃癌组织化疗前后、60例胃良性疾病组织和50例正常对照组织PBMC中Th1和Th2类细胞因子mRNA表达。结果:正常对照及胃良性疾病组PBMC中Th1和Th2类细胞因子基因阳性率差异无统计学意义,P>0.05。胃癌组PBMC中IL-4、IL-6和IL-10 mRNA阳性率显著高于正常对照和胃良性疾病组(P<0.05),且与胃癌的分化程度及临床分期相关,P<0.05;IFNγ-仅3例阳性表达(3/102),IL-2有7例阳性表达,显著低于正常对照和胃良性疾病组;胃癌组化疗有效者PBMC中Th1类细胞因子阳性率增加,Th2类细胞因子的阳性率则减低,P<0.05;化疗无效者PBMC中Th1和Th2类细胞因子mRNA阳性率较化疗前差异无统计学意义,P>0.05。结论:检测Th1和Th2类细胞因子mRNA的表达对评价胃癌患者的免疫状况和疾病进展及指导临床对患者进行免疫调节治疗有一定的应用价值。
Objective: To investigate the changes of Th1 and Th2 cytokine mRNA expression in peripheral blood mononuclear cells (PBMCs) of patients with gastric cancer and its clinical significance. Methods: The expressions of Th1 and Th2 cytokines in PBMC of 102 gastric cancer tissues before and after chemotherapy, 60 gastric benign disease tissues and 50 normal control tissues were detected by RT-PCR. Results: There was no significant difference in the positive rates of Th1 and Th2 cytokines in PBMC between normal control group and gastric benign disease group (P> 0.05). The positive rates of IL-4, IL-6 and IL-10 mRNA in PBMC of gastric cancer group were significantly higher than those of normal control group and gastric benign disease group (P <0.05), and were correlated with the differentiation degree and clinical stage of gastric cancer (P <0.05) - Positive expression of Th1 cytokines in peripheral blood mononuclear cells was observed in only 3 cases (3/102) and 7 cases of IL-2, which were significantly lower than those in normal controls and benign gastric diseases. (P <0.05). The positive rate of Th1 and Th2 cytokines in PBMCs with chemotherapy ineffectiveness was not significantly different from that before chemotherapy (P> 0.05). Conclusion: Detecting the expression of Th1 and Th2 cytokines mRNA has some value in evaluating the immune status and disease progression of patients with gastric cancer and guiding clinical immunotherapy for patients.