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目的:3种梅毒检测方法比较,选择适合特殊人群梅毒感染状况的筛查方法。方法:采用ELISA法进行抗体检测,并与TRUST方法检测结果进行比较,阳性样本再用TPPA法进行确证。结果:经TPPA确认阳性的282件样本中,ELISA方法检出281件阳性,检出率为99.6%,TRUST方法检出194件阳性,检出率为68.8%,差异具显著统计学意义;ELISA方法检测样本OD值的高低与TPPA符合率有着显著差异,与TRUST符合率也有显著差异。结论:ELISA法能满足特殊人群梅毒感染状况筛查,但对OD值较低的阳性样本应再以TPPA确认。
OBJECTIVE: To compare the detection methods of three kinds of syphilis and choose the screening method suitable for the infection of syphilis in special population. Methods: Antibody was detected by ELISA and compared with TRUST method. The positive samples were confirmed by TPPA method. Results: Of the 282 samples positive for TPPA, 281 were positive by ELISA, the detection rate was 99.6%, and 194 were positive by TRUST, the detection rate was 68.8%. The difference was statistically significant There was a significant difference between the method of detecting the OD value of the sample and the coincidence rate of TPPA, and the coincidence rate of TRUST was also significantly different. Conclusion: The ELISA method can meet the screening needs of syphilis in special population, but the positive samples with lower OD should be confirmed by TPPA.