论文部分内容阅读
目的建立高效液相色谱-电化学法(HPLC-ECD)检测24 h 尿甲基福林(MN)和甲基去甲福林(NMN)的方法,并通过与血尿儿茶酚胺(CA)、尿 CA 的比较验证 MN、NMN 对嗜铬细胞瘤诊断的优越性。方法使用 MCX 固相萃取柱对 MN、NMN 进行提取,采用 HPLC-ECD 法加以检测,计算组内、组间变异和样品回收率。用此方法对104例非嗜铬细胞瘤高血压患者和5例嗜铬细胞瘤患者进行 MN、NMN 检查,同时对所有病例的血 CA、24 h 尿 CA 进行检测。结果 MN 的组内、组间变异系数和回收率分别为5.9%、7.5%、91.1%;NMN 的组内、组间变异和回收率分别为6.3%、6.6%、88.5%。病例组的 MN、NMN、血 CA、尿 CA 均为阳性;对照组的 MN 和 NMN 无假阳性,而血 CA、尿CA 分别出现15、14例假阳性。结论本研究建立了 HPLC-ECD 快速准确检测24 h 尿 MN、NMN 的方法,MN、NMN 是目前临床检验嗜铬细胞瘤的最佳指标。
OBJECTIVE To establish a HPLC-ECD method for the detection of 24 h urinary methylfuran (MN) and methyl norfurien (NMN) The comparison verifies the superiority of MN and NMN in the diagnosis of pheochromocytoma. Methods MN and NMN were extracted by MCX solid phase extraction column and detected by HPLC-ECD method. Intra-and inter-group variation and sample recovery were calculated. Using this method, 104 cases of non-pheochromocytoma hypertensive patients and 5 cases of pheochromocytoma patients MN, NMN examination, all cases of blood CA, 24 h urinary CA test. Results The intra-and inter-group variation coefficient and recovery of MN were 5.9%, 7.5% and 91.1%, respectively. The intra-and inter-group variation and recovery rate of MN were 6.3%, 6.6% and 88.5% respectively. MN, NMN, blood CA and urine CA in the case group were all positive; MN and NMN in control group had no false positive, while blood CA and urine CA appeared 15 and 14 false positive respectively. Conclusion This study established a rapid and accurate HPLC-ECD detection of 24 h urinary MN, NMN, MN, NMN is the best clinical indicators of pheochromocytoma.