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目的 通过检测肝细胞癌 (hepatocellularcarcinoma ,HCC)微卫星不稳定 (microsatellitein stability,MSI)和杂合性缺失 (lossofheterozygosity ,LOH)发生频率 ,探讨上述遗传学改变与HCC临床病理的关系。方法 选择 5个微卫星多态性标记对 32例HCC进行了MSI与LOH分析。结果 32例HCC中有 14例出现MSI,其中有 6例出现 2个位点MSI,后者癌肿转移率明显低于其他的组 (P =0 0 0 1) ,在所检 5个位点中D1S484和TP5 3MSI发生频率最高 ,而D9S16 0 4和D8S5 5 5遗传学改变多为LOH且发生频率较低。结论 MSI是部分HCC发生、发展进程中一个重要的遗传学改变 ,尤其在无癌肿转移的肿瘤中。D1S484和TP5 3是HCC中对MSI敏感的检测位点。
Objective To investigate the frequency of microsatellite instability (MSI) and loss of heterozygosity (LOH) in hepatocellular carcinoma (HCC) and to explore the relationship between the above genetic changes and clinicopathological features of HCC. Methods Fifteen microsatellite polymorphism markers were used to analyze MSI and LOH in 32 HCC patients. Results MSI was found in 14 of 32 HCC cases, of which 6 showed MSI at two sites and the latter showed significantly lower rates of metastasis of cancer than those in the other groups (P = 0 0 01) In D1S484 and TP5 3MSI the highest frequency of occurrence, and D9S160 4 and D8S5 5 5 genetically modified mostly LOH and the frequency of occurrence is low. Conclusion MSI is an important genetic change in the development and progression of some HCCs, especially in cancers without metastasis. D1S484 and TP5 3 are MSI-sensitive detection sites in HCC.